Keywords
antioxidant activity
2,2-diphenyl-1-picrylhydrazyl (DPPH)
tannins
How to Cite
Abstract
Objective – to estimate the antiradical scavenging ability of extracts of plants of Galega officinalis L. and G. orientalis Lam., depending on phase of growing in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG).
Material and methods. Plant material of this investigation was two species of Galega L. (G. officinalis and G. orientalis) collected from experimental collection of Cultural Flora Department of NBG: GOFSV (G. officinalis, spring vegetation), GOFB (G. officinalis, budding stage), GOFF (G. officinalis, flowering stage), GOFSR (G. officinalis, seed ripening stage), GORSV (G. orientalis, spring vegetation), GORB (G. orientalis, budding stage), GORF (G. orientalis, flowering stage), GORSR (G. orientalis, seed ripening stage). The antiradical activity of methanol, ethanol and aqueous extracts, based on the discoloration reaction on the solution of DPPH (2,2-diphenyl-1-picrylhydrazyl free radical), was determined by spectrophotometric method according to Brandt-Williams et al. Biochemical preparation and analyze was carried out in the Institute of Biodiversity Conservation and Biosafety, the Slovak University of Agriculture in Nitra (Slovak Republic) and NBG. The total content of tannins was determined by titrimetric method according to Krischenko (water extracts with indigo carmine were titrated by permanganate solution).
Results. We determined that methanol extracts of Galega officinalis plants had antiradical activity in range from 19.39 % (GOFB) to 95.18 % (GOFSR), ethanol extracts – from 11.24 % (GOFB) to 92.87 % (GOFF), and water extracts – from 28.64 % (GOFSR) to 74.63 % (GOFF). Methanol extracts of Galega orientalis plants had antiradical activity from 20.20 % (GORSR) to 91.72 % (GORB), ethanol extracts – from 11.74 % (GORSR) to 84.74 % (GORF), and water extracts – from 22.90 % (GORSV) to 77.72 % (GORF). The total content of tannins for G. officinalis was in range of 1.22 to 4.17 % and for G. orientalis – from 1.55 to 4.42 % during vegetation.
Conclusions. Plant raw material of two Galega L. species is potential source of antioxidants. During vegetation antiradical activity of plant extracts of Galega officinalis exhibited 11.24–95.18 % and Galega orientalis – 11.74–91.72 % depending on extract and phase of growing in conditions of NBG. Generative organs such as flowers and fruits had less content of tannins than vegetative.
References
Lupak, M.I., Khohla, M.R., Hachkova, G.Ya., Kanyuka, A.P., Klymyshyn, N.I., Chajka, Ya.P., Skibitska, M.I. and Sybirna, N.O. (2015), Bezalkoloidna frakciya ekstraktu kozlyatnyka likarskoho (Galega officinalis L.) poperedgaje oksydatyvnyi stress v umovah eksperymentalnoho dibetu [The alkaloid-free fraction from Galega officinalis L. extract prevents oxidative stress under experimental diabetes mellitus]. Ukrainian Biochemical Journal, vol. 87, N 4, pp. 78—86. Moda access: http://dx.doi.org/10.15407/ubj87.04.078
Vergun, O.M., Shymanska, O.V. and Rakhmetov, D.B. (2012), Biohimichna harakterystyka roslyn rodu Galega L. v Pravoberegnomu Lisostepu Ukrajiny [Biochemical characteristic of Galega L. species in Right-Bank of Forest-Steppe of Ukraine]. Visnyk Odesskoho nacionalnoho universytetu. Seriya Biologiya [Odessa National University Gerald. Biology], vol. 17, vyp.3, N 28, pp. 43—50.
Krischenko, V.P. (1983), Metody ocenki kachestva rastitelnoy produkcii [Methods for evaluating of quality of plant production]. Moscow: Kolos, 192 p.
Khohla, M., Hachkova, G. and Sybirna, N. (2016), Porivnyanna hypoglikemichnoi dii vodnyh ekstraktiv, suspenziy yakona ta bezalkaloidnoi frakcii ekstraktu galegi likarskoi [A comparison of hypoglycemic action of yacons water extracts, suspensions and alkaloid-free fraction from Galega officinalis L. extract]. Visnyk Lvivskoho universytetu. Seria biologichna [Herald of Lviv University. Ser. Biologian], vol. 73, pp. 421—428.
Amarowicz, R. (2007), Tannins: the new natural antioxidants? European Journal of Lipid Science Technology, vol. 109, pp. 549—551. Moda access: http://dx.doi.org.10.1002/ejit.200700145
Balazentiene, L. and Mikulioniene, S. (2006), Chemical composition of galega mixtures silages. Agronomy Research, vol. 4, N 2, pp. 483—492.
Balazentiene, L. and Spruogis, V. (2011), Experience of fodder galega (Galega orientalis Lam.) and traditional fodder grasses use for forage production in organic farm. Veterinary Medicine and Zootechnic, vol. 56, N 78, pp. 19—26.
Baradaran, A. (2016), A short look to the nephroprotective impacts of metformin. Toxicologia Persa, vol. 1, N 1, pp. e01. Moda access: http://toxicolpersa.com
Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995), Use of a free radical method to evaluate antioxidant activity. LWT — Food Science and Technology, vol. 28, N 1, pp. 25—30.
Casi, K. and Defronzo, R.A. (1998), Metformin: a review of its metabolic effect. Diabetes Review, vol. 6, pp. 89—131.
Kiselova, Y., Ivanova, D., Chervenkov, T., Gerova, D., Galunska, B. and Yankova, T. (2006), Correlation between the in vitro antioxidant activity and polyphenol content of aqueous extracts from Bulgarian herbs. Phytotherapy Reaserch, vol. 20, pp. 961—965. Moda access: http://dx.doi.org//10.1002/ptr.1985
Shojaee, S.S., Vahdati, A., Assaei, R. and Sapeh rimanesh, M. (2015), Effect of Galega officinalis leaf powder and Trigonella foenum-graecum seed powder on blood glucose levels and weight gain in diabetes mellitus rat model. Comparative Clinical Pathology, vol. 24, pp. 145—148. Moda access: http://dx.doi.org//10.1007/s00580-013-1873-7
Meripold, H., Tamm, U., Tamm, S., Vosa, T. and Edesi, L. (2017), Fodder galega (Galega orientalis Lam.) grass potential as a forage and bioenergy crop. Agronomy Research, vol. 15, N 4, pp. 1693—1699. Moda access: https://doi.org/10.15159/AR.17.021
Mardani, S., Nasri, N., Rafieian-Kopaei, M. and Hajian, S. (2013), Herbal medicine and diabetic kidney disease. Journal of Nephropharmacology, vol. 2, N 1, pp. 1—2.
Lemus, I., Garcia, R., Delvillar, E. and Knop, G. (1999), Hypoglycaemic activity of four plants used in Chilean popular medicine. Phytotherapy Research, vol. 13, pp. 91—94.
Modak, M., Dixit, P., Londhe, J., Ghaskadbi, S. and Deva sagayam, T.P.A. (2007), Indian herbs and herbal drugs used for the treatment of diabetes. Journal of Clinical Biochemical Nutrition, vol. 40, pp. 163—173.
Karakas, P.F., Yildirim, A. and Turker, A. (2012), Biological screening of various medicinal plant extracts for antibacterial and antitumor activities. Turkey Journal of Biology, vol. 36, pp. 641—652. Moda access: http://dx.doi.org/10.3906/biy-1203-16
Khodadadi, S. (2016), Administration of Galega officinalis L. in experimental and clinical investigations: a narrative review. Annals of Research of Antioxidants, vol. 1, N 1, pp. e03.
Leporatti, M.L. and Ivancheva, S. (2003), Preliminary comparative analysis of medicinal plants used in the tra ditional medicine of Bulgarian and Italy. Journal of Ethnopharmacology, vol. 87, pp. 123—142.
Marinova, G. and Batchvarov, V. (2011), Evaluation of the methods for determination of the free radical scavenging activity by DPPH. Bulgarian Journal of Agricultural Science, N 1, pp. 11—24.
Ozbucak, T.B., Erturk, O. and Akcin, O.E. (2005), An ecological, anatomical and microbiological investigation on species Galega officinalis L. (Leguminosae) in some localities of Turkey. Pakistan Journal of Biological Sciences, vol. 8, N 9, pp. 1215—1220.
Tusevski, O., Kostovska, A., Iloska, A., Trajkovska, L. and Simic Gadzovska, S. (2014), Phenolic production and antioxidant properties of some Macedonian medicinal plants. Central European Journal of Biology, vol. 9, N 9, pp. 888—900. Moda access: http://dx.doi.org/10.2478/s11535-014-0322-1
Piluzza, G. and Bullitta, S. (2011), Correlations between phenolic content and antioxidant properties in twenty-four plant species of traditional ethnoveterinary use in the Mediterranean area. Pharmaceutical Biology, vol. 49 (3), pp. 240—247. Moda access: https://doi.org/10.3109/13880209.2010.201083
Rafieian-Kopaei, M., Baradaran, A. and Rafieian, M. (2013), Plants antioxidants: from laboratory to clinic. Journal of Nephropathology, vol. 2, N 2, pp. 152—153. Moda access: http://dx.doi.org/10.12860/JNP.2013.26
Palma Schwertner, A., Ricci, A., Parpinello, G.P. and Versari, A. (2017), Rapid screening method to assess tannin antioxidant activity in food-grade botanical extract. Italian Journal of Food Sciences, vol. 29, pp. 559—564.
Shymanska, O., Vergun, O., Rakhmetov, D. and Fishchenko, V. (2017), The content of photosynthetic pigments in the leaves of Galega officinalis L. and Galega orientalis Lam. cultivars. Agrobiodiversity for improving nutrition, health and life quality, vol. 1, pp. 398— 403. Moda access: http://dx.doi.org/10.15414/agro bio diver sity.2017.2585-8246.398-403
Kumar, S., Saini, M., Kumar, V., Prakash, O., Arya, R., Rana, M. and Kumar, D. (2012), Traditional medicinal plants curing diabetes: a promise for today and tomorrow. Asian Journal of Traditional Medicines, vol. 7, N 4, pp. 178—188.
Umashanker, M. and Shruti, S. (2011), Traditional Indian herbal medicine used as antipyretic, antiulcer, anti-diabetic and anticancer: a review. International Journal of research in pharmacy and chemistry, vol. 1 (4), pp. 1152—1159.
Varis, E. (1986), Goat’s rue (Galega orientalis Lam.) a potential pasture legume for temperate conditions. Journal of Agricultural Sciences of Finland, vol. 58, pp. 83—101.
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